Pioneering Innovation Through Products and People

Manju Gupta

Fellow, Dow AgroSciences

Planting the Seed

In both her personal and professional life, Manju has been a pioneer. Now, one of the most senior scientists in Dow, she looks to pass her pioneering spirit on.

Manju Gupta was just 14 years old when she knew she wanted to be a scientist. “In high school, I enjoyed biology and I was fascinated with learning about plants and animals,” Manju said. “I’m grateful that my father insisted that I get an education, even though his father didn’t think this was necessary. For my grandfather, girls got married and that was enough. But my father supported my passion for science.”

His support allowed Manju to transform her talent and dedication into a career as a leading researcher in agricultural science that has impacted people’s lives around the world. “One of the first areas I worked on was transferring genes from algae into rice plants, which eliminates the need for rice field fertilization,” Manju said. “This helps farmers save time and money so they can feed more people. Now it has become my life’s passion to improve crops for sustained agriculture and better human health.”

Fielding Tough Questions, Finding Answers

Throughout her career as a scientist and researcher, Manju has found that there are three keys to solving tough problems: persistence, observation and collaboration. She applied these principles to find a critical solution during the development of Herculex-RW™, an industry-leading product that makes plants resistant to crucial insects.

Herculex-RW was created through a collaboration between several companies and their teams of researchers. During field trials in the product development stage, Manju and the other researchers were confronted with a major obstacle – highly undesirable red leafs in corn plants. The other researchers focused on work in the lab, but Manju took a different approach.

“Very few were observing the problem in the field,” she said. “I was working in the lab, but realized what was happening in the field was a critical missing dimension. I communicated with DowAgroSciences’ field scientist, Jim Bing, every day. These conversations led me to hypothesize that the expression of certain genes was the root cause of the red leaf issue. This proved true. We then corrected the problem, which cleared the way for product development.”

Awards

Dow AgroSciences Excellence in Science Award, 2005

Eleven Achievement in Crop Genetics and Technology Awards (ACGT) for the Seed, Traits and Oils Function, 2000-2009

Twenty Dow patent awards, 2007-2014

Post-doctoral Fellowship, Max-Planck Institute, Cologne, Germany, 1981-1983

Commonwealth Fellowship to study abroad (England), 1979-1981

Associations

American Society of Plant Biologists

Crown Gall Society

US 8592645B2 (2013): Engineered zinc finger proteins targeting plant genes involved in fatty acid biosynthesis

US 7,288,643 (2007); US20110099662; US 7449564 (2008); US 7435807 (2008); US 7417132 (2008); US 7514544 (2009), US 7989607 (2011); . 64003J-US-DIV[2] (2013); US 8901378 (2014): Corn Event TC1507 and methods for detection thereof

US 7323556 (2008); US 7897342 (2011); US 7888023 (2011); US 7888495 (2011); US 7956246 (2011); US 7875429 (2011); US 7695914 (2011); US 7875430 (2011); US 7696341 (2011); US 7932033 (2011); 20110030086; US 7932033 (2011); US 8592653 (2013); RE43373: Corn event DAS-59122-7 and methods for detection thereof

EP1613725B1 (PCT/US2004/003852; International application WO2004/072259): Altered Fad3 genes in Brassica and the molecular marker-assisted detection thereof

US PSP 20140123: Zea mays regulatory element and their uses of

US PSP 74276: Maize regulatory element and uses thereof

US PSP 74276: Zea mays regulatory elements and uses thereof

US PSP 74485: Zea mays regulatory elements and uses thereof

US PSP 74253: Zea mays metallothionein-like regulatory elements and uses thereof

US PSP 76018: Methods and compositions for recombination; A-gene deficient strains of Agrobacterium tumefaciens

US PSP 73951: Use of agrobacterium genes VirD1 and VirD2 to reduce vector backbone DNA sequences and increase single copy integrations in transgenic plants generated through agrobacterium-mediated transformation

US PSP 73951: Use of agrobacterium genes VirD1 and VirD2 to reduce vector backbone DNA sequences and increase single copy integrations in transgenic plants generated through agrobacterium-mediated transformation

US PSP 69893: Novel maize ubiquitin promoters

Provisional Application 75663: Novel maize ubiquitin promoters and their use

Provisional Application 75664: Novel maize ubiquitin promoters and their use

Provisional Application 75665: Novel maize ubiquitin promoters and their use

Provisional Application 75666: Novel maize ubiquitin promoters and their use

PCT Application 72751: High-throughput DNA fragment assembly

PCT Application 72908: Use of a maize untranslated region for transgene expression in plants

Provisional Application 224882: Constructs and methods for expressing transgenes using regulatory elements from ubiquitin genes

US 20140090113: Engineered transgene integration platform (ETIP) for gene targeting and trait stacking

IDM 72288: Fad3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks

US 0090112: Fad2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks

US 2013039309: Targeted modification of malate dehydrogenase

US2013/0104257 A1: Construction and methods for expressing transgenes using a brassica constitutive bidirectional promoter

Provisional Application 61617252: Construct and method for expression of multiple transgenes in plants

Provisional Application 61641956: Construct and method for expression of multiple transgenes in plants II

Provisional Application 61675929: High-throughput DNA fragment assembly

Provisional Application 61684255: Use of a maize untranslated region for transgene expression in plants

US 12/64683: Construct and method for synthetic bidirectional plant promoter ubi1

US 13674712: Construct and method for synthetic bidirectional SCBV plant promoter

US 20110167521: Engineered zinc finger proteins targeting plant genes involved in fatty acid biosynthesis

EP2205749A1 and WO/2009/042164A1: Engineered zinc finger proteins targeting 5-Enoylpyruvyl Shikimmate-3-Phosphate Synthase genes

US 2011/0151441: Endpoint TaqMan methods for determining zygosity of corn comprising TC1507 events

US2006/0248611/A1: Altered FAD2 and FAD3 genes in brassica and the altered molecular marker – assisted detection thereof

Kumar S, AlAbed D, Chen W, DeSloover D, Whitteck J, Asberry A, Bennett S, Wang X, Rangaswamy M, Wright T, Gupta M. 2015. A Combinatorial Bidirectional Promoter and Bicistronic Approach for Coordinated Multi-Gene Expression in Corn. Plant Mol Biol, 87:341-353.

Kumar S, AlAbed D, Worden A, Novak S, Wu H, Ausmus C, Beck M, Robinson H, Hemingway D, Lee R, Skaggs N, Wang L, Marri P, Gupta M. 2015. A Modular Gene Targeting System for Sequential Transgene Stacking in Plants. Jn of Biotechnology, 207:12-20.

Gupta, M., DeKelver, RC., Palta, A., Clifford, C., Gopalan, S., Miller, JC., Novak, S., Desloover, D., Gachotte, D., Connell, J., Flook, J., Patterson, T., Robbins, K., Rebar, EJ., Gregory, PD., Urnov F.D., and Petolino, J. F. 2012. Transcriptional activation of Brassica napus β-ketoacyl-ACP synthase with an engineered zinc finger protein transcription factor reduces saturated fatty acid content in leaves and seeds. Plant Biotechnology Journal, 10: 783-791.

Gupta, M., Nirunsuksiri, W., Schulenberg, G., Hartl, T., Novak, S., Bryan, J., Vanopdorp, N., Bing, J., Thompson, S. 2008. A non-PCR-based Invader® assay quantitatively detects single-copy genes in complex plant genomes. Molecular Breeding, 21:173:181.

Hu, X., Sullivan-Gilbert, M., Gupta, M., Thompson, S.A. 2007. G-to-A mutation at a 5’ splice site of fad3c caused impaired splicing in a low linolenic mutant of canola (Brassica napus L.) Plant Biotechnology, 24: 397 – 400.

Xueyi, H., Sullivan-Gilbert, M., Gupta, M., Thompson, SA. 2006. Mapping of the loci controlling oleic and linolenic acid contents and development of fad2 and fad3 allele-specific markers in canola (Brassica napus L.) Theor Appl Genet. 113(3):497-507.

Gupta, M. and Raghav, Ram. 2004. Development of Genetically modified agronomic crops. Page 219-241. In: Genetically Modified Animals, Microbes and Plants in Biotechnology. Edited by Parekh, S.R. Humana Press, Totowa, New Jersey.

Horne, E.C., Kumpatla, S.P., Patterson, K.A., Gupta M. and Thompson, S.A. 2004. Improved high-throughput sunflower and cotton genomic DNA extraction and PCR fidelity. Plant Molecular Biology Reporter 22(1): 83-84.

Kumpatla, S.P., Manley, M.K., Horne, E.C., Gupta, M. and Thompson, S.A. 2004. An improved enrichment procedure to develop multiple repeat classes of cotton microsatellite markers. Plant Molecular Biology Reporter 22(1): 85-86.

Staub, J.E., Serquen, F.C. and Gupta, M. 1996. Genetic markers, map construction and their application in plant breeding. Horticulture Science., 31:729-741.

Gupta, M., Chyi, Y. S., Romero-Severson, J. and Owen, J. L. 1994. Amplification of evolutionarily diverse genomes using single primers of simple-sequence repeats. Theor. Appl. Genet., 89:998-1006.

Gupta, M. and Hoo, B. 1991. Entire maize chloroplast genome is stably maintained in a yeast artificial chromosome. Pl. Mol. Biol., 17:361-369.

Gupta, M., Chourey, P.S., Still, P.E., and Burr, B. 1988. cDNAs of the two non-allelic sucrose synthase genes in maize: Cloning, expression and characterization, and molecular mapping of the sucrose synthase-2 gene. Pl. Mol. Biol., 10:215-224.

Gupta, M., Shepherd, N.S., Bertram, I., and Saedler, H. 1984. Repetitive sequences and their organization on genomic clones of Zea mays. EMBO J., 3:133-139.

Shepherd, N.S., Schwarz-Sommer, Z.S., Blumberg vel Spalve, J., Gupta, M., Wienand, U., and Saedler, H. 1984. Similarity of the Cin1 repetitive family of Zea mays to eukaryotic transposable elements. Nature, 307:185-187 6.

Gupta, M., Bertram, I., Shepherd, N.S. and Saedler, H. 1983. Cin1, a family of dispersed repetitive elements in Zea mays. Mol. Gen. Gen., 192:373-377.

Saedler, H., Bonas, U., Deumling, B., Gupta, M., Hahlbrock, K., Harrison, B.J., Kreuzaler, F., Peterson, P.A., Reif, J., Schwarz-Sommer, Zs, Shepherd, N.S., Sommer, H., Ubben, D., and Wienand, U. 1983. In: Transposable elements in plants. Genetic Rearrangement (Eds. Charter, K.F., Cullis, C.A., Hopwood, D.A., Johnston, A.A.W.B., and Woolhouse, H.W.), pp. 107-115, Croom Helm Press, London and Canberra.

Gupta, M. and Carr, N.G. 1983. Isolation of polysomes from the Nostoc sp. MAC and translation of messenger RNA in a heterologous cell-free system. J. Gen. Microbiol., 129:2359-2366.

Gupta, P.P., Gupta, M., and Schieder, O. 1982. Correction of nitrate reductase defect in auxotrophic plant cells through protoplast-mediated intergeneric gene transfer. Mol. Gen. Genet., 188:378-383.

Gupta, P.P., Schieder, O., and Gupta, M. 1984. Intergeneric nuclear gene transfer between somatically and sexually incompatible plants through asymmetric protoplast fusion. Mol. Gen. Genet., 197:30-35.

Gupta, M. and Carr, N.G. 1981. Enzyme activities related to cyanophycin metabolism in heterocysts and vegetative cells of Anabaena spp. J. Gen. Microbiol., 125:10-17.

Gupta, M. and Carr, N.G. 1981. Detection of glutamate synthase in heterocysts of Anabaena sp. strain 7120. J. Bacteriol., 148:980-982.

Gupta, M. and Carr, N.G. 1981. Enzymology of arginine metabolism in heterocyst-forming cyanobacteria. FEMS Microbiol. Letts., 12:179-181.

Kumar, H.D. and Gupta, M. 1978. Effects of cyclic nucleotides on morphogenesis in Nostoc muscorum. Arch. Microbiol., 119:1983-1986.

Agrawal, M. and Kumar, H.D. 1978. Effects of 7-azatryptophan on heterocyst differentiation in Anabaena doliolum Bharadwaja. Proc. Indian Acad. Sci., 87B:31-39.

Agrawal, M. and Kumar, H.D. 1977. Cobalt toxicity and its possible mode of action in blue-green alga Anacystis nidulans. Beitrag Zur Biol. Pflanzen, 53:157-163.

Agrawal, M. and Kumar, H.D. 1976. Response of Anacystis nidulans to the growth regulators ethrel and IAA. Proc. of Physiol. of Microorganisms (ed. K.S. Bilgrami), Today and Tomorrow Press, New Delhi, pp. 135-140.

Gupta M. 2014. Invited talk, “Deploying ZFNs in Plants,” Plant Genome Stability and Change, Monterey, California.

Kumar S, AlAbed D, Worden A, Lee R, Bennett S, Novak S, Wu H, Ausmus C, Beck M, Robinson H, Hemingway D, Foulk D, Skaggs N, Chen W, Wang L, Lutz L, Marri P, Evans C, Gupta M. 2014. Oral

presentation, “A Modular and Flexible Gene Targeting System for Multigenerational Transgene Stacking in Plants,” 35th Crown Gall Conference, Purdue University, Indiana.

Chennareddy S, Cicak T, Clark L, Bennett S, Russell S, Novak S, Pareddy D, Sardesai N, Sarria R, Gupta M. 2014. Poster presentation, “A Brassica napus Bi-directional Promoter Exhibits Differential Leaf-Expression Patterns in Soybean Transgenic Lines Generated Using Agrobacterium-Mediated Transformation,” 35th Crown Gall Conference, Purdue University, Indiana.

Gupta M, Novak S, Urnov F, Dekelver RC, Gopalan S, Miller M, Rebar E, Clifford C, Zhang L, Russell S, Connell J, DeSloover D, Crosley R, Walker A, Bauer T, Gachotte D, Flook J, McNew B, Patterson T, Petolino J. 2011. Oral presentation, “Gene Regulation Using Engineered Zinc Finger Protein Transcription Factors,” Genome Stability and Change EMBO Conference, France.

Merlo D, Russell S, Crosley R, AlAbed D, Jayne S, Blue R, Worden A, Gupta M. 2011. Oral presentation, “Increase in Corn Transformation Efficiency as a Result of Agrobacterium Strain Modification,” 32nd Crown Gall Conference. October 28-30, Middleton, Wisconsin.

Gupta M, Novak S, Urnov F, Dekelver RC, Gopalan S, Miller M, Rebar E, Clifford C, Zhang L, Russell S, Connell J, DeSloover D, Crosley R, Walker A, Bauer T, Gachotte D, Flook J, McNew B, Patterson T, Petolino J. 2010. Invited talk, “Gene Regulation using Engineered Zinc Finger Protein Transcription Factors,” Molecular and Cellular Biology of Soybean Conference, North Carolina.

Shukla, V., Bauer, T., Arnold, N., Gao, Z., McCaskill, D., Mitchell, J., Rowland, L., Simpson, M., Worden, S., Yau, K., Gupta, M., Urnov, F., Miller, J., Rock, J., Moehle, E., DeKelver, R., Doyon, Y., Rebar, E., Collingwood, T., Zhang, L. 2008. “Zinc-finger Nuclease Mediated Gene Targeting in Maize: Genome Editing via Homologous Recombination,” ASPB, Merida, Mexico.

Gopalan, S., Novak, S., Miller, J. Gupta, M., Rebarb, E., Gregory, P., Urnov, F., Reddy, S. and Petolino, J. 2007. Designed Zinc Finger Nuclease-Mediated In Vitro Cleavage of Genes of a Dicot Species. Plant DNA Repair and Recombination, Presquile de Giens, France.

Xueyi Hu, Mandy Sullivan-Gilbert, Tom Kubik, Jason Danielson, Nathan Hnatiuk, Wesley Marchione, Manju Gupta, Katherine Armstrong and Steven Thompson. 2007. “Development of Molecular Markers Specific to the Ogura Fertility Restorer Gene Rfo in Canola (Brassica napus L.). 2007. Proceedings of the 12th International Rapeseed Congress II, Pp 314-316, Wuhan, China.

Hu, X., Sullivan-Gilbert, M., Gupta, M., Ram, R., and Thompson, S. 2005. “Cryptic Splicing Of Fad3 Pre-mRNA In A Low Linolenic Mutant Of Canola,” The American Society of Plant Biologists conference, Seattle, Washington.

Sun, Y., Schnelle, K., Thompson, M., Greene, T., Gupta, M., Shukla, V. and Rubin-Wilson, B. 2005. “Global Transcript Analysis of the Transition from Mitotic Cell Division to Endoreduplication During Maize Endosperm Development,” The American Society of Plant Biologists, Seattle, Washington.

Gupta, M., Bing, J., Higgins, L., Schulenberg, G., Thompson, S., Wang, J., Xu, D., and Zhong, G.-Y. 2004. “Post-Transcription Gene Silencing and its Reversibility in Maize Hybrids,” The American Society of Plant Biologist, (p. 184), Orlando, Florida.

Gupta, M., Nirunsuksiri, W., Schulenberg, S., Hartl, T., Thompson, S.A. 2002. “The Invader Operating System: An Innovative Technology for Many Agricultural Applications,” The American Society of Plant Biologists, Denver, Colorado.

Hartl., T., Nirunsuksiri, W., Gupta., M., Thompson, S. A., Roeven, R., and De Arruda, M. 2001. “Determination of Copy Number in Transgenic Plants Using the Invader Operating System,” Beyond the Human Genome, Baltimore, Maryland.

Hu, X.Y., Sullivan, M.L., Gupta, M. and Thompson, S.A. 2003. “Cloning of Fad2 and Fad3 Genes and Development of Gene-Specific Markers for High Oleic and Low Linolenic Acids in Canola,” Proceedings of the11th International Rapeseed Congress, Copenhagen, Denmark.

Hu, X.Y., Sullivan, M.L., Gupta, M., Ram, R. and Thompson, S.A. 2004. “Fad2 and Fad3 Mutations and Mutation-Specific Markers for High Oleic and Low Linolenic Acids in Canola,” Plant and Animal Genome XII, San Diego, California.

Hu, X.Y., Hundley, M.L., Wang, L., Chyi, Y.S., Gupta, M., and Thompson, S.A. 2001. “Molecular Mapping of Loci Controlling Oleic and Linolenic Content In Canola,” Plant and Animal Genome IX, San Diego, California.

Kumpatla, S.P., Shah, M.R., Gupta, M., Thompson, S.A. and Ram, R. 2004. “Approaches for Rapid Development of SSRs and SSR-Based SNP Markers in Cotton,” International Cotton Genome Initiative ICGI-2004 Workshop, Hyderabad, India.

Shah, M.R., Kumpatla, S.P., Gupta, M. and Thompson, S.A. 2004. “SSR-Based SNP Discovery: An Economical Approach for the Development of SNP Markers in Cotton,” Plant and Animal Genome Meeting XII, San Diego, California.

Shah, M.R., Kumpatla, S.P., Gupta, M. and Thompson, S.A. 2003. “The SNP-IT™ Platform: An Efficient Single Nucleotide Polymorphism Detection Technology for Plant Molecular Genetics,” Plant and Animal Genome Meeting XI, San Diego, California.

Horne, E.C, Kumpatla, S.P., Patterson, K.A., Gupta, M. and Thompson, S.A. 2003. “Improving PCR Fidelity in Genomic DNA Samples Derived from Plant Tissues Rich in Secondary Compounds,” Plant and Animal Genome Meeting XI, San Diego, California.

Hundley, M., Hu., X., Scott, T., Ernst, C., Cui, C., Gupta, M., and Thompson, S.A. 2002. “Utilization of SSR Markers to Determine Genetic Similarity and Heterotic Association in Helianthus annus,” Plant Animal Genome X, San Diego, California.

Ernst, C. A., Mason, M., Gupta, M., and Thompson, S. A. 2001. “Utilization of SSR Markers to Determine Genetic Similarity and Heterotic Associations in Zea may,” Proceedings of Plant and Animal Genome IX, San Diego, California.

Ernst, C.A., Clayton, K. A., Ren, R., Mickelson-Young, L. A., Frank, C., Robideau., C. R., Gupta, M., and Thompson, S. A. 2000. “Utility of Marker Assisted Selection for Introgression of Commercially Important Genes into Elite Germplasm,” Proceedings of the 42nd Annual Maize Genetics Conference, Coeur D’Alene, Idaho.

Kumpatla, S.P., Horne, E.C., Shah, M., Gupta, M. and Thompson, S.A. 2002. “Development of SSR Markers Toward Genetic Mapping in Cotton,” The Third International Cotton Genome Initiative (ICGI) Workshop, Nanjing, People’s Republic of China. Also published in: Cotton Science (China) Vol. 14, supplement: 26 (English edition).

Horne, E.C., Kumpatla, S.P., Gupta, M. and Thompson, S.A. 2001. “High Throughput Development of Simple Sequence Repeat Markers in Cotton,” Plant and Animal Genome Meeting IX, San Diego, California.

Kumpatla, S.P., Manley, M.K., Horne, E.C., Gupta, M. and Thompson, S.A. 2000. “Large-scale Development of Multiple Repeat Classes of Microsatellite Markers in Cotton Using an Efficient Selective Enrichment Procedure,” Plant and Animal Genome Meeting VIII, San Diego, California.

Hu., X., Wang., L., Hundley, M. L., Chyi, Y.-S., Gupta, M., and Thompson, S. A. 2000. “Molecular Mapping of Loci Controlling Oleic and Linolenic Content in Canola,” DAS/PBL Meetings, San Diego, California.

Manley, M. K., Gupta, M., Schulenberg, G. A., Pitas, J. M., Litcher, S.J., Robideau, C. R., Mason, M. E., Burr, B. and Chyi, Y.S. 1998. “Similar Distribution and Coverage of AFLP Markers on the Corn Genome with Two Restriction Enzyme Combinations,” Plant Genome VI, San Diego, California.

Romero-Severson, J., Gupta, M. and Burr, B. 1997. “Relative Distribution of SSR, AFLP and RFLP Markers in a Consensus Map of Maize,” Plant Genome V, San Diego, California.

Gupta, M., Manley, M. and Murray M. 1996. “Comparison of ABI 373A and ABI 377 DNA Sequencers for AFLP Analysis,” Plant Genome IV, San Diego, California.

Gupta, M. 1995. “Pre-amplification Restriction Enzyme Digestion of Complex Genomes Increases the Utility of Microsatellite-Based SPAR,” Plant Genome III, San Diego, California.

Gupta, M., Romero-Severson. J. and Murray, M. 1995. “Detection of AFLPs with ABI Fluorescent Technology: The Rapid Construction of Maize Maps,” Plant Genome III, San Diego, California.

Zaitlin, D., DeMars, S. and Gupta, M. 1992. “Linkage of a Second Gene for NCLB Resistance to Molecular Markers in Maize,” Maize Genetics Cooperation Newsletter, vol. 72.

Uyeda, C.M., Gupta, M., and Steffensen, D.M. 1992. “A Putative Maize Telomeric Repeat is Dispersed in the Genome as Detected by in situ Hybridization,” Maize Genetics Cooperation Newsletter, vol. 72.

Gupta, M. and Filner, P. 1991. “Microsatellites Amplify Highly Polymorphic DNA Bands in SPAR of Plant DNA,” Proceedings of the International Society of Pl. Mol. Biol.,Tucson, Arizona.

Owen, J. and Gupta, M. 1991. “DNA Analysis for Animal Breeders,” Proceedings of the Fortieth Annual National Breeders Roundtable, Saint Louis, Missouri.

Gupta, M., Park, F.D., Hoo, B., Fromme, M., Zaitlin, D., Chyi, Yan-San, Banks, W.D., and Filner, P. 1989. “Identification of the RFLP Markers for the Ht1 Gene in Maize by Comparison of Inbreds and their Ht1-Conversions,” Maize Genet. Cooperation Newsletter, vol. 63, p. 112.

Chyi, Yan-San, Gupta, M., Fromme, M.J., Park, F.D., Hoo, B., Zaitlin, D., Banks, W.D., Perkins, J., and Filner, P. 1989. “Identification of RFLP Markers for the rhm gene in Maize,” Maize Genet. Cooperation Newsletter, vol. 63, pp. 112-113.

Gupta, M., Chourey, P.S., and Still, P.E. 1987. “Cloning, Immunoselection and Characterization of cDNA Clones of the Two Non-Allelic Sucrose Synthase Genes,” Maize Genet. Cooperation Newsletter, 61:56-57.

Shepherd, N.S., Gupta, M., Schwartz-Sommer, Zs., Wienand, U., Deumling, B., and Saedler, H. 1983. “The Cin1 Repeat of Zea mays and Zea mexicana,” Maize Genet. Cooperation Newsletter, 57:158.

Scheider, O., Gupta, P.P., Gupta, M., Krumbiegel, G., Hein, T. and Steffen, A. 1983. “The transfer of genomes, ” Protoplastes de Plantes Superieres de Nouvelles. Perspectives en Biologie Vegetale., Strasbourg, France, p. 19.

Gupta, P.P., Gupta, M. and Schieder, O. 1985. “Intergeneric Nuclear Gene Transfers by Asymmetric Fusion of Plant Protoplasts,” First International Congress of Plant Molecular Biology, Savannah, Georgia, p. 106.

Carr, N.G., Gupta, M., Rylah, J., Mckie, J. 1982. “The Role of Cyanobacterial Reserve Materials as Dynamic Reservoirs in Nutrient Assimilation,” IV International Symposium of Photosynthetic Prokaryotes, Bordeaux, France.

Gupta, M. and Carr, N.G. 1980. “Arginine Formation and Cyanophycin Metabolism in Anabaena,” Proc. Membranes and the Microbial Cell Cycle: Their Diverse Roles and Synthesis, Society of General Microbiology, London, vol. 8, pt. 1.